Negin Kouchak Dezfuli
Tehran Medical Branch - Islamic Azad University, Iran
Title: Comparison of the biocompatibility of ProRoot MTA, MTA Plus and Retro MTA using an MTT assay study
Biography
Biography: Negin Kouchak Dezfuli
Abstract
The purpose of this in vitro study is to evaluate and compare the cytotoxicity of three commercially available root-ending materials,MTA Plus, Pro root MTA and Retro MTA, at different storage times after mixing on human periodontal fibroblast using a MTT method. Varied concentrations (3, 6, 12, 25, 50 mg/ml) of the fresh and set root-ending materials (ProRoot MTA, MTA Plus and Retro MTA) were placed in adjacent flasks of human periodontal fibroblast in DMEM medium within 96-well plates. Cellular viability was evaluated using a MTT assay after 24, 48, and 72 h of initial mixing. The results were analyzed with 1-way ANOVA. The results showed that there was no significant difference between the biocompatibility of MTA Plus, and that of Pro root MTA or Retro
MTA (P>0.05). Furthermore, no significant difference was observed between different time intervals for each group (P>0.05). The current in vitro study showed almost similar biocompatibility for ProRoot MTA and MTA Plus and Retro MTA.