Alaa Elaraby
Alexandria University, Egypt
Title: The effect of different finish lines on marginal and internal fit of a ceramic crown fabricated by CAD/CAM system
Biography
Alaa Elaraby has completed his Bachelor of Dental Surgery from Alexandria University, Faculty of Dentistry, PhD from Nagasaki University, and Postdoctoral studies from Nagasaki University, School of Dentistry. He created the phase diagram of Gold–Copper Pseudo-binary system at Nagasaki University, Japan. He established and created the curriculum of the Doctorate program in Dental Materials at College of Dentistry, King Saud University. He was the Director of Dental Biomaterials courses for undergraduate and postgraduate students at University of Alexandria and King Saud University. He has published more than 30 papers in local and international peer reviewed dental journals and has been serving as an Editorial Board Member of the Saudi Dental Journal (Section Editor in Restorative Dentistry).
Abstract
This study evaluates the marginal discrepancy of CAD/CAM ceramic coping of two different finish line preparations. Two master steel dies were prepared, one with rounded shoulder (RS), and the other with chamfer (C). CEREC 3D Sirona CAD/CAM system and Software Version 2.80 R2400 were used in this study. The optical camera was fixed in a distance of 10 cm and 90 degree to take the same optical capture for each sample. CEREC cylinder pointed bur was used to cut the Ivoclar Vivadent ProCAD Milling Blocks to fit on the dies. Twenty copings were prepared, ten CAD/CAM ceramic coping from each finish line preparation. Each coping with its corresponding die was sectioned longitudinally using ISOMET 2000 PRECISION SAW. The marginal discrepancy was then evaluated in Travelling Microscope. The data of marginal discrepancy in micrometer (μ) were subjected to two way analysis of variance using SPSS version 15. The mean value of marginal discrepancy was 135.48 μm and 85.7 μm for chamfer group (C) and rounded shoulder group (R) respectively. Furthermore, there was a significant difference in the axial fitting on the occlusal side(occlusal adaptation) of the crowns between the chamfer and shoulder groups (p<0.05). Shoulder group showed the lowest mean value of 95.84 μ ± 15.02, while chamfer recorded the highest value of 137 μ ±13.09. The results showed that there was a significant difference between the two groups. It was concluded that marginal fit of CAD/CAM all-ceramic crowns with rounded shoulder finish line had better adaptation than chamfer finish line. The adaptation of CAD/CAM crowns obtained with chamfer finish line was not clinically accepted.
Qian Yajie
Nanjing University, China
Title: Effects of NF-κB inhibitor on the regulation function of cigarette smoke extract to human β defensins in the immortalized human oral mucosal epithelial cell line
Biography
Qian Yajie completed her Master’s degree from Nanjing University. She is now a Clinical Doctor at Nanjing Stomatological Hospital Medical School of Nanjing University. Since the beginning of the graduate student stage, she has been engaged in Oral Medicine research. So far, she has published a paper as the first author, in Cellular Physiology and Biochemistry. She contributed in the other three papers, which were all included in SCI. In recent years, she has participated in several research projects, and won a number of awards, including “New Technological Introduction Award†from the Health Department of Jiangsu Province in 2014.
Abstract
Cigarette smoke increases the susceptibility to oral mucosal infection and is a risk factor for malignant transformation. Cigarette smoke is a mixture of thousands of toxic components generated upon the burning or heating of tobacco leaves. The toxic components first interface with the immune system at the oral mucosal surfaces. NF-κB is a new member of the family of transcription factors, which positively regulate the expression of many genes involved in inflammatory and other responses, including human β defensins (hBDs). The hBD family is one type of cationic antimicrobial peptides that can be secreted by epithelial cells. Among hBD family, hBD-1, -2 and -3 are critical members of the defense system of oral mucosal epithelium. We treated immortalized human oral mucosal epithelial (Leuk-1) cells with various concentrations of cigarette smoke extract (CSE) for 24 h. Western blotting and immunofluorescence assays were performed to study CSE-induced alteration of NF-κB, and P-NF-κB protein expression. The change of hBDs expression were tested using qPCR and ELISA. And then we adopted BAY 11-7082, a specific inhibitors of NF-kB, to inhibit the activation of NF-kB signalling. Different concentrations of NF-κB inhibitor NF BAY 11-7082 were treated to Leuk-1. Then 10 μM BAY 11-7082 was added to the cell culture 24 h before the addition of 4% CSE for 24 h. Leuk-1 cells were treated with 0.5% DMSO as a mock-treated control. Real-time PCR and ELISA were performed to detect the mRNA levels and secretion of hBD-1, -2,and -3, respectively. In this research, we found CSE treatment suppressed NF-κB expression and activated P-NF-κB expression in Leuk-1 cells. The mRNA and secretory levels of hBD-1 and -3 were down-regulated by CSE, while the mRNA and secretory level of hBD-2 were up-regulated by CSE. The BAY 11-7082 treatment significantly abrogated the inhibitory effect of CSE on hBD-1 mRNA expression and release, BAY 11-7082 treatment remarkably reversed the induced effect of CSE on hBD-2 mRNA level and release,while BAY 11-7082 treatment clearly removed the inhibitory effect of CSE on hBD-3 mRNA level and release. The present study indicated that CSE regulated the expression levels of hBDs via down-regulated NF-κB in oral mucosal epithelial cells.